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Dermal Biopsy Harvesting Requirements

Dermal Biopsy Harvesting Requirements

DB is collected from a donor site after general or localized anesthesia. The dermis can be obtained by either punch biopsy or incisional biopsy. A punch biopsy is made through the skin after local anesthesia, from the epidermis down to the hypodermis, and the tissue punch is inserted into a sterile collection tube containing sterile transport medium. An incisional biopsy is made through the epidermis after local anesthesia and a piece (or strip) of dermis is removed after which the area is closed with sutures. The sample is placed into a sterile container with sterile transport medium. Maximal sample size should be no more than 25 cm2 from any single site. If the site is warm and red to the touch, indicating a possible infection, the sample should be considered contaminated and there should be a high index of suspicion for an infection of the sample area. Appropriate steps should be taken to discard the DB sample and to rule out localized infection prior to reattempt at DB biopsy for ASC therapy.